Cell Sorting and Solid Phase Extraction
This project focuses on the use of an integrated microdevice that combines sedimentation-based cell sorting and solid phase extraction (SPE) of DNA from the sorted cells. The microdevice (figure-left), fabricated using standard photolithographic techniques, is designed with a domain for cell sorting and two separate SPE regions. A mixture of sperm and epithelial cells are separated according to their physical properties (figure-right). Sperm cells are then lysed on-chip, followed by isolation and purification of its DNA fractions. Following cell separation and SPE on the microdevice, DNA amplification and separation are performed using conventional laboratory methods. This work represents a major step towards the development of a fully integrated microdevice capable of genetic DNA analysis.
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Contact Landers Research Group
jpl5e@virginia.edu (434) 243-8658 375, 379, 395 Chemistry Building McCormick Road Charlottesville, Va 22904 |
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