UNIVERSITY OF VIRGINIA
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        • Acoustic Differential Extraction
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        • Enhanced Sperm Cell Recovery
        • Monolith Stationary Phase for Microfluidic DNA Purification
        • Nucleic Acid Purification in a Two-Stage, Dual-Phase Microchip
        • Large Volume Reduction Solid Phase Extraction
        • Plastic SPE Microdevices
      • Polymerase Chain Reaction (PCR) >
        • Infrared PCR
        • Microwave PCR
      • Label-Free Optical Methods for DNA and Cell Quantification
      • DNA Extraction and PCR Amplification
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      • Passive Valving
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      • A Fully Integrated Microfluidic Genetic Analysis Device for the Detection of Blood Cancers
      • Electric Field-Flow Fractionation for DNA Concentration
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Microwave PCR

Dielectric heating is commonly used, most notably in the form of a microwave oven. Our group in collaboration with Dr. N. Scott Barker in UVA’s department of Electrical and Computer Engineering is developing a microfluidic microwave heating device for small volumes of solution. Using microstrip transmission lines focused microwave power can be delivered directly to a microliter chamber containing solution. The figure shows an example of our chip-based microwave heating device. With about 1 W of non-resonant microwave power (for comparison a microwave oven uses ~ 1000 W) we rapidly heat buffer to boiling and can vary temperature by adjusting the microwave frequency, or delivered power. The figure also shows an example of temperature control by varying the applied microwave frequency. We are currently applying this technology to the polymerase chain reaction which requires thermocycling between two or three temperatures for up to 30 cycles to amplify a desired DNA fragment. 

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Contact Landers Research Group
jpl5e@virginia.edu
(434) 243-8658
375, 379, 395 Chemistry Building
McCormick Road
​Charlottesville, Va 22904
UVA Chemistry Department
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  • Home
  • Previous Research
    • Centrifugal Microdevices >
      • PET Chips
    • Clinical Diagnostics and Forensic Analysis >
      • Cell Sorting and Solid Phase Extraction >
        • Acoustic Differential Extraction
        • Isolation of Circulating Tumor Cells
        • Enhanced Sperm Cell Recovery
        • Monolith Stationary Phase for Microfluidic DNA Purification
        • Nucleic Acid Purification in a Two-Stage, Dual-Phase Microchip
        • Large Volume Reduction Solid Phase Extraction
        • Plastic SPE Microdevices
      • Polymerase Chain Reaction (PCR) >
        • Infrared PCR
        • Microwave PCR
      • Label-Free Optical Methods for DNA and Cell Quantification
      • DNA Extraction and PCR Amplification
    • Fluidic Control >
      • Passive Valving
    • Genetic Analysis >
      • A Fully Integrated Microfluidic Genetic Analysis Device for the Detection of Blood Cancers
      • Electric Field-Flow Fractionation for DNA Concentration
      • Acousto-Optic Tunable Fiber
    • Narcotics and Explosives Colorimetric Detection
  • People
  • Publications
  • Collaborators
  • Contact
  • Conferences