Large Volume Reduction Solid Phase Extraction
Often times in forensic cases large volumes are required to elute or solubilize a blood sample from various surfaces including clothing and walls. In contrast, microdevices are characterized by small sample volumes. This raises the need for a sample processing step that would reduce the volume of the forensic sample, providing both a crude purification and sample concentration effect. These investigations focus on a volume reduction microdevice utilizing various solid phases including chitosan-coated-silica particles (charge switch binding) and magnetic silica particles (hydrogen bonding). A comparison of the extraction efficiency of the two phases will be investigated, in addition to optimizing the integration with a second, silica based SPE step. Later work will also involve integration with other processing steps including PCR, ME, and fluorescence detection.