UNIVERSITY OF VIRGINIA
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Enhanced Sperm Cell Recovery from Cotton Swabs for Rape Kit Analysis

Regardless of the method utilized for the separation of vaginal and sperm cell DNA, the overall effectiveness of the procedure is ultimately dependent on the efficiency with which material can be eluted and recovered from a cotton swab. The issue is especially important with swab samples containing low numbers of sperm cells, where any loss makes it even more difficult to obtain a profile of the perpetrator.  This project focuses on the development of improved methods for cell elution from a cotton matrix. Several alternative methods of intact cell removal have been investigated; including the use of cellulase-based enzyme mixtures [1], as well as the exclusive use of detergents [2]. These methods can be utilized in conjunction with or to circumvent conventional differential extraction. 

1. Voorhees, J.C., Ferrance, J.P., Landers, J.P. Enhanced elution of sperm from cotton swabs via enzymatic   digestion for rape kit analysis. J Forensic Sci 2006; 51(3):574-9.
2. Norris, J.V., Manning, K., Linke, S.J., Ferrance, J.P.,Landers, J.P.  Expedited, chemically-enhanced sperm cell recovery from cotton swabs for rape kit analysis. J Forensic Sci 2007; 52(4):800-5 

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Contact Landers Research Group
jpl5e@virginia.edu
(434) 243-8658
375, 379, 395 Chemistry Building
McCormick Road
​Charlottesville, Va 22904
UVA Chemistry Department
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  • Home
  • Previous Research
    • Centrifugal Microdevices >
      • PET Chips
    • Clinical Diagnostics and Forensic Analysis >
      • Cell Sorting and Solid Phase Extraction >
        • Acoustic Differential Extraction
        • Isolation of Circulating Tumor Cells
        • Enhanced Sperm Cell Recovery
        • Monolith Stationary Phase for Microfluidic DNA Purification
        • Nucleic Acid Purification in a Two-Stage, Dual-Phase Microchip
        • Large Volume Reduction Solid Phase Extraction
        • Plastic SPE Microdevices
      • Polymerase Chain Reaction (PCR) >
        • Infrared PCR
        • Microwave PCR
      • Label-Free Optical Methods for DNA and Cell Quantification
      • DNA Extraction and PCR Amplification
    • Fluidic Control >
      • Passive Valving
    • Genetic Analysis >
      • A Fully Integrated Microfluidic Genetic Analysis Device for the Detection of Blood Cancers
      • Electric Field-Flow Fractionation for DNA Concentration
      • Acousto-Optic Tunable Fiber
    • Narcotics and Explosives Colorimetric Detection
  • People
  • Publications
  • Collaborators
  • Contact
  • Conferences